Real-Time PCR System

Sample capacity

96

Reaction volume

10-50 μl

Thermal cycle technology

Peltier

Max. Heating/Cooling rate

6.0° C/s

Heating temperature range

4 – 100 °C

Temperature accuracy

± 0.2°C

Temperature uniformity

±0.2℃ @60℃ ,  ±0.3℃ @95℃

Temperature gradient setting range

30–100°C

Temperature gradient difference setting range

1 – 36°C

Detection system

Excitation light source

4/6 monochrome high efficiency LEDs

Detection device

PMT

Detection mode

Time-resolved signal separating technology

Excitation/detection wavelength range

455-650nm/510-715nm

Fluorescent channels

4/6 channels

Supported dye

FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3,   JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ

Sensitivity

Single copy gene

Resolution

1.33 folds copy number difference can be distinguished   in single-plex qPCR

Dynamic range

10 orders of magnitude copies